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KMID : 0370220220660060324
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Yakhak Hoeji
2022 Volume.66 No. 6 p.324 ~ p.330
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Effect of Hyperoside on Phorbol Ester-induced Gene Expression of Mucin and NF-κB Signaling in Cultured Human Airway Epithelial Cells
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Lee Hyun-Jae
Lee Choong-Jae
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Abstract
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We investigated whether hyperoside, an anti-inflammatory natural product, significantly affects phorbol 12-myristate 13-acetate (PMA)-induced airway expression of MUC5AC encoded mucin. Confluent NCI-H292 cells werepretreated with hyperoside for 30 min, then stimulated with PMA for 24 h or the indicated periods. MUC5AC mucinmRNA expression was measured by RT-PCR. MUC5AC mucin protein expression was quantitated by ELISA. To elucidatethe mechanism of hyperoside action, we investigated the effect of hyperoside on PMA-induced NF-κB signaling bywestern blot analysis. The results were as follows: (1) Hyperoside significantly inhibited MUC5AC mucin proteinproduction and down-regulated MUC5AC mucin mRNA expression; (2) Hyperoside inhibited PMA-induced activation(phosphorylation) of inhibitory kappa B kinase (IKK), thereby inhibiting phosphorylation and degradation of inhibitorykappa Bα (IκBα); (3) Hyperoside inhibited PMA-induced nuclear translocation of nuclear factor kappa B (NF-κB) p65,thereby down-regulating MUC5AC mucin gene expression in NCI-H292 cells. These results suggest that hyperoside canregulate mucin gene expression by airway epithelial cells through regulation of the NF-κB signaling pathway.
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KEYWORD
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Respiratory, Hyperoside, Mucin
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